Semi-prep HPLC 37-SPH100

Semi-prep is equipped with 4 sets of high pressure pumps providing a flow rate variance of 50 ml / min or 100 ml / min and a UV-Vis detector with a wavelength range of 190 to 800 nm. Equipped with a mixing chamber to enhance homogenization and reciprocating pump for consistent flow. With a column size of 10 x 250 mm and particle size of 5 µm or 10 µm, the retention, resolution and selectivity is optimized.

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Pump Specifications
Model No SPH-A10 SPH-A11 SPH-A12 SPH-A13
Flow rate range 0.001 ~ 50 ml / min 0.001 ~ 50 ml / min 0.001 ~ 100 ml / min 0.001 ~ 100 ml / min
Maximum pressure 30 MPa 30 MPa 15 MPa 10 MPa
Flow rate accuracy ± 0.35 ± 0.5 ± 0.5 ± 0.5
Flow rate repeatability % ≤ 0.1 ≤ 0.15 ≤ 0.1 ≤ 0.15
Column size 10 x 250 mm 10 x 250 mm 10 x 250 mm 10 x 250 mm
Sample loading 35 ~ 60 mg 35 ~ 60 mg 35 ~ 60 mg 35 ~ 60 mg
Pressure pulsation 1 % at 10 MPa 1.5 % at 10 MPa 1.5 % at 10 MPa 1.5 % at 10 MPa
Flow rate repeatability % ≤ 0.1 ≤ 0.15
Gradient accuracy % ± 2 ± 2 ± 2 ± 2
Gradient repeatability % ≤ 0.2 ≤ 0.2 ≤ 0.2 ≤ 0.2
Dimension 465 x 315 x 180 mm 455 x 265 x 190 mm 465 x 315 x 180 mm 465 x 315 x 180 mm
Power 150 W 150 W 150 W 150 W
Power supply 90 V ~ 240 V/ 50 Hz 90 V ~ 240 V/ 60 Hz 90 V ~ 240 V/ 50 Hz 90 V ~ 240 V/ 60 Hz
Weight 11.6 kg 11.2 kg 11.6 kg 11.6 kg
Detector Specifications
Model No SPH-A20 SPH-A21 SPH-A22
Wavelength range 190 to 800 nm 190 to 800 nm 190 to 800 nm
Wavelength accuracy ± 0.5 nm ± 1 nm ± 0.75 nm
Wavelength repeatability 0.15 nm 0.3 nm 0.2 nm
Band width 8 nm 8 nm 8 nm
Optical path Semi flow / analytical Semi flow / analytical Semi flow / analytical
Baseline noise ( Static ) Au ≤ ± 0.35 x 10 -5 ≤ ± 0.5 x 10 -5 ≤ ± 0.5 x 10 -5
Baseline drift ( Static ) Au / h ≤ 1 x 10 -4 ≤ 1.5 x 10 -4 ≤ 1.5 x 10 -4
Minimum detection limit ≤ 4 x 10 -8 ≤ 4 x 10 -8 ≤ 4 x 10 -8
Power 150 W 150 W 150 W
Power supply 90 V / 50 ~ 60 Hz 90 V / 50 ~ 60 Hz 90 V / 50 ~ 60 Hz
Dimension 465 x 315 x 180 mm 465 x 315 x 180 mm 460 x 265 x 190 mm
Weight 9.5 kg 9.5 kg 9.2 kg
Column Specifications
Inner diameter 100 mm , 20 mm , 30 mm , 50 mm
Column length 100 mm , 150 mm , 250 mm , 300 mm
Particle size 5 µm , 10 µm
  • Solvent flow rate is 2 ~ 4 ml / min
  • Selectable sample of volume loop by injector according to end user need
  • Normal and reverse phase compatibility
  • Compatibility for medium and low pressure application
  • PEEK pump head and detector for bio-purification
  • Compatible for fraction collector and fraction valve
  • Built-in UV , RI , DAD
  • Degasser ( optional )

Accessory Quantity
Autosampler 1
Fraction collector ( 144 tubes , 12 mm test tubes ) 1
Fraction valve ( 6 channel ) 1

Used in up scaling of small scale production, purification of small amount of substances (mg) , in pharmaceutical analysis, food nutrient analysis of amino acids and sugars,chemical product purity analysis

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